Coding

Part:BBa_K3454043:Design

Designed by: Zhiyu Fang   Group: iGEM20_ShanghaiTech_China   (2020-10-27)


AsCas12a_RVRR_6xHis_MBP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1393
    Illegal PstI site found at 4864
    Illegal PstI site found at 5062
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2083
    Illegal PstI site found at 1393
    Illegal PstI site found at 4864
    Illegal PstI site found at 5062
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
    Illegal BglII site found at 5052
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1393
    Illegal PstI site found at 4864
    Illegal PstI site found at 5062
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1393
    Illegal PstI site found at 4864
    Illegal PstI site found at 5062
    Illegal NgoMIV site found at 4596
    Illegal AgeI site found at 3493
    Illegal AgeI site found at 3964
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79
    Illegal BsaI.rc site found at 2236
    Illegal BsaI.rc site found at 3898
    Illegal SapI.rc site found at 1486


Design Notes

This is designed with MBP and his tag that can help AsCas12a mutant expression, folding and purification.


Source

It comes from expression of plasmid DNA(contains designed mutation ) of E.coli

References